Do you have to list lack of standing as an affirmative defense or lose the ability to use it?

[trudey]

If I just file a general denial and not use "lack of standing" as an affirmative defense, can I still force the JDB to have witnesses be served within 150 miles and then challenge the standing when the witnesses are not at the right location for service? In the post below, it seems like all the OP did was file a general denial and no affirmative defenses and was still successful in challenging standing.